Rusfertide

Research Reagent · Laboratory Use Only

What does research show about rusfertide for polycythemia vera?

Rusfertide is a hepcidin-mimetic peptide from Protagonist Therapeutics and Takeda that controls hematocrit in polycythemia vera by restricting iron availability for red blood cell production. The Phase 2 REVIVE trial (NEJM, 2024) showed a 60% clinical response rate versus 17% for placebo, eliminating the need for therapeutic phlebotomy in most responders. The Phase 3 VERIFY trial confirmed these results (77% vs 33% response), and the FDA accepted Takeda's New Drug Application with Priority Review, targeting a decision around Q3 2026.

Scientific AbstractPMID 38381675 · 2024

Rusfertide (PTG-300, TAK-121) is an injectable peptide mimetic of hepcidin, the body's master iron-regulatory hormone, developed by Protagonist Therapeutics and Takeda. By restricting iron availability for erythropoiesis, rusfertide controls hematocrit in polycythemia vera (PV), a chronic myeloproliferative neoplasm, independent of cytoreductive therapy. In the Phase 2 REVIVE trial (N Engl J Med, Feb 2024; PMID 38381675; NCT04057040), rusfertide eliminated the need for therapeutic phlebotomy and maintained hematocrit below 45% in phlebotomy-dependent PV patients, with a 60% response rate versus 17% for placebo during the randomized-withdrawal phase.

The Phase 3 VERIFY trial (NCT05210790, n=293) confirmed superiority, with rusfertide plus standard of care roughly doubling clinical response rates over placebo plus standard of care (77% vs 33%) and eliminating phlebotomy eligibility during weeks 20-32, meeting its primary endpoint and all four key secondary endpoints. Based on this data package plus four-year REVIVE/THRIVE extension safety and efficacy data, the FDA accepted Takeda's New Drug Application and granted Priority Review, Breakthrough Therapy, Orphan Drug, and Fast Track designations, with a PDUFA target action date in Q3 2026.

Mechanistic Research SummaryCurated from PubMed

This data is for laboratory research purposes only. Not for human or animal consumption.


What is Rusfertide?

Rusfertide (PTG-300, TAK-121) is a synthetic hepcidin-mimetic peptide developed by Protagonist Therapeutics in collaboration with Takeda. It is being investigated as a first-in-class therapy for polycythemia vera (PV), a chronic myeloproliferative neoplasm characterized by excess red blood cell production (erythrocytosis). Unlike the other metabolic and neurological peptides in this database, rusfertide's target pathway is iron regulation and erythropoiesis rather than GLP-1/GIP signaling or GHRH/GABA pathways.


Mechanism of Action

Rusfertide mimics hepcidin, the liver-derived master regulator of systemic iron homeostasis. By binding ferroportin and blocking iron export from cells, it restricts the iron supply available for red blood cell production, lowering hematocrit without direct cytotoxic or myelosuppressive effects — a mechanistically distinct alternative to phlebotomy and cytoreductive drugs (hydroxyurea, interferon) in PV management.


Observed Clinical Results

  • Phase 2 REVIVE trial (NEJM, Feb 2024; PMID 38381675; NCT04057040): In the randomized-withdrawal phase, 60% of rusfertide-treated patients maintained hematocrit control without phlebotomy versus 17% on placebo (P=.002); mean maximum hematocrit fell from 50.0% pre-treatment to 44.5% on rusfertide.
  • Phase 3 VERIFY trial (NCT05210790, n=293): Rusfertide plus standard of care produced a 77% clinical response rate (freedom from phlebotomy eligibility, weeks 20-32) versus 33% for placebo plus standard of care — met primary endpoint and all four key secondary endpoints.
  • Long-term extension (REVIVE/THRIVE, ~4 years of data): Supports durable safety and efficacy, submitted as part of the NDA package.

Regulatory Status

Investigational — FDA accepted Takeda's NDA and granted Priority Review (accepted March 2, 2026) with a PDUFA goal date in Q3 2026 (~August 2026). Also holds Breakthrough Therapy, Orphan Drug, and Fast Track designations. Not a compounding-pharmacy peptide; if approved, rusfertide would be a branded injectable specialty drug, not 503A-eligible.

Clinical Research Parameters
2 trials3 human studies

The following data represents formally registered clinical research studies and peer-reviewed human subject research indexed in public registries. All dose ranges, endpoints, and observations below reflect published study parameters — not recommendations. For research reference only.

ClinicalTrials.gov ↗
NCT04057040
COMPLETEDPhase IIn=70

REVIVE: A Study of PTG-300 in Patients With Polycythemia Vera

International, three-part Phase 2 trial of rusfertide (PTG-300) in phlebotomy-dependent polycythemia vera. Part 1 was a 28-week open-label dose-finding period; Part 2 was a 12-week double-blind, randomized-withdrawal period. During Part 2, a clinical response (hematocrit control without phlebotomy) was observed in 60% of rusfertide-treated patients versus 17% of placebo patients. Mean maximum hematocrit fell from 50.0% pre-treatment to 44.5% during Part 1. Published in the New England Journal of Medicine, February 2024.

Study Interventions
Rusfertide (PTG-300), Placebo
Primary Endpoints
Proportion of responders during randomized withdrawal period (weeks 29-41)
Study Period
2019-10 → 2023-12
NCT05210790
ACTIVE NOT RECRUITINGPhase IIIn=293

VERIFY: A Phase 3 Study of Rusfertide in Patients With Polycythemia Vera

Global, randomized, double-blind, placebo-controlled Phase 3 trial of rusfertide plus standard of care versus placebo plus standard of care in phlebotomy-dependent polycythemia vera. Met primary endpoint and all four key secondary endpoints: 77% of rusfertide-treated patients achieved a clinical response (absence of phlebotomy eligibility, weeks 20-32) versus 33% of placebo patients. Results presented at ASH 2025 and ASCO 2025/2026; forms the primary basis of Takeda's FDA New Drug Application, accepted with Priority Review in March 2026 (PDUFA goal date Q3 2026).

Study Interventions
Rusfertide (PTG-300), Placebo, Standard of care (phlebotomy, cytoreductive therapy)
Primary Endpoints
Proportion of responders (hematocrit control without phlebotomy, weeks 20-32)
Study Period
2022-04 → 2027-12

All data presented on this page is for laboratory research purposes only. Rusfertide is referenced here as a research reagent. This page does not constitute medical advice, clinical guidance, or endorsement of any compound for human or animal use. All referenced studies are available via PubMed (PMID: 38381675) and the DOI-linked journal publication. Researchers must consult applicable institutional and regulatory frameworks before conducting any protocols.

Rusfertide in the Research Blog